The 2-Minute Rule for thr77 login
The 2-Minute Rule for thr77 login
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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene is noticed in many tumors, which results in chromosome instability, and therefore may add to tumorigenesis. This protein was located to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a critical position in marketing cell-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are actually explained. Two added splice variants had been reported but thorough nucleotide sequence information and facts just isn't still obtainable.
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Blocking peptides are peptides that bind exclusively into the target antibody and block antibody binding. These peptide ordinarily includes the epitope regarded from the antibody.
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This guideline outlines further more doable optimization of cellular and immunoassay parameters to make sure the very best benefits are...
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This information outlines more possible optimization of mobile and immunoassay parameters to make sure the absolute get more info best results are...
Antibodies certain to the blocking peptide now not bind to your epitope around the focus on protein. By comparing the staining from your blocked antibody vs . the antibody on your own, you can see which staining is unique.
Goal Information and facts Cyclin E belongs for the really conserved cyclin loved ones, whose customers are characterized by a remarkable periodicity in protein abundance in the cell cycle. Cyclins operate as regulators of CDK kinases. Unique cyclins show distinctive expression and degradation styles which lead to your temporal coordination of each and every mitotic celebration. Cyclin E forms a complex with and functions like a regulatory subunit of CDK2, whose action is needed for cell cycle G1/S transition. This protein accumulates in the G1-S phase boundary and is degraded as cells development as a result of S period.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...
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